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KMID : 0606920100180030280
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Biomolecules & Therapeutics
2010 Volume.18 No. 3 p.280 ~ p.285
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Chitosan Increases α6 Integrinhigh
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Shin Dong-Wook
Park Soo-Nam
Shim Joong-Hyun
Kim Yoon-Kyung
Son Eui-Dong
Yang Seung-Ha
Jeong Hye-Jin
Kim Han-Kon
Lee Seok-Yong
Noh Min-Soo
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Abstract
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Glycosaminoglycans (GAGs) and chitosan have been used as matrix materials to support the dermal part of skin equivalent which is used for both pharmacological and toxicological evaluations of drugs potentially used for dermatological diseases. However, their biological roles of GAGs and chitosan in the skin equivalent are still unknown. In the present study, we evaluated whether GAGs and chitosan directly affect keratinocyte stem cells (KSCs) and their transit-amplifying cells (TA cells). Among supporting matrix materials, chitosan significantly increased the number of α6 integrinhigh/CD71high human keratinocyte TA cells by 48.5%. In quantitative real-time RT-PCR analysis, chitosan significantly increased CD71 and CD200 gene transcription whereas not α6 integrin. In addition, the level of the gene transcription of both keratin 1 (K1) and K10 in the chitosan-treated human keratinocytes was significantly lower than those of control, suggesting that chitosan inhibit keratinocyte differentiation. We also found that N-acetyl-D-glucosamine (NAG) and β-(1-4)-linked D-glucosamine (D-glc), two components of chitosan, have no effect on the expression of CD71, K1, and K10, suggesting that each monomer component of chitosan is not enough to regulate the number of epidermal keratinocyte lineage. Conclusively, chitosan increases keratinocyte TA cell population which may contribute to the cellular mass expansion of the epidermal part of a skin equivalent system.
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KEYWORD
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Chitosan, Keratinocyte stem cells, Transit-amplifying cells, CD71, K1, K10
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